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Promega goscript reverse transcriptase
Goscript Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscript+reverse+transcriptase/goscripttm+reverse+transcription+system/pmc12269245-32-8-16
Average 90 stars, based on 1 article reviews
goscript reverse transcriptase - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Reverse Transcription Polymerase Chain Reaction:

Article Title: Identification of a novel hypovirulence-inducing ourmia-like mycovirus from Fusarium solani causing ginseng ( Panax ginseng ) root rot
Article Snippet: This dsRNA was then treated with enzymes including S1 Nuclease and Recombinant DNase I (TaKaRa, Dalian, China) before electrophoresis on a 1.0% agarose gel. .. For RT-PCR detection, first-strand cDNA was synthesized using GoScript Reverse Transcriptase (Promega, United States), followed by PCR amplification with specific primers (2-4F and 2-4R), as detailed in . ..

Synthesized:

Article Title: Identification of a novel hypovirulence-inducing ourmia-like mycovirus from Fusarium solani causing ginseng ( Panax ginseng ) root rot
Article Snippet: This dsRNA was then treated with enzymes including S1 Nuclease and Recombinant DNase I (TaKaRa, Dalian, China) before electrophoresis on a 1.0% agarose gel. .. For RT-PCR detection, first-strand cDNA was synthesized using GoScript Reverse Transcriptase (Promega, United States), followed by PCR amplification with specific primers (2-4F and 2-4R), as detailed in . ..

Reverse Transcription:

Article Title: Identification of a novel hypovirulence-inducing ourmia-like mycovirus from Fusarium solani causing ginseng ( Panax ginseng ) root rot
Article Snippet: This dsRNA was then treated with enzymes including S1 Nuclease and Recombinant DNase I (TaKaRa, Dalian, China) before electrophoresis on a 1.0% agarose gel. .. For RT-PCR detection, first-strand cDNA was synthesized using GoScript Reverse Transcriptase (Promega, United States), followed by PCR amplification with specific primers (2-4F and 2-4R), as detailed in . ..

Article Title: Retinal Angiogenesis in Methamphetamine Self-Administration Rats.
Article Snippet: Relative levels of protein expression normalized with β-actin (Santa Cruz Biotechnology, Dallas, TX, USA) were analyzed by ImageJ, v1.52a (National Institutes of Health, Bethesda, MD, USA). .. To determine mRNA expression levels, total RNA annealed with an oligo dT15 probe was reverse-transcribed to cDNA with GoScript Reverse transcriptase (Promega, Madison, WI, USA). .. The relative mRNA expression levels were estimated using a Bio-Rad CFX Fast Real-Time PCR System and iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Super-enhancer-mediated circRNAs exhibit high splicing circularization diversity and transcriptional activity.
Article Snippet: .. GoScript Reverse Transcriptase (Promega) was used to synthesize the first-strand complementary DNA. ..

Article Title: C1QL1 inhibits breast cancer through the HSP90α/VCP-ERS/UPR axis.
Article Snippet: TRIzol reagent (Invitrogen) was used to isolate total RNA from cells or tissues in accordance with the manufacturer’s instructions. .. Promega GoScript reverse transcriptase (Promega) was used to reverse-transcribe aliquots containing 1 μg of total RNA to 20 μl cDNA. .. The reaction mixture for quantitative PCR with reverse transcription (qRT–PCR) analysis was supplemented with 2 μl cDNA and SYBR green (Invitrogen).

Article Title: EIF4A3-induced circPRKAR1B promotes esophageal squamous cell carcinoma progression through binding PKM2 to regulate NF-κB induced CCL3 secretion
Article Snippet: Total RNA was extracted from ESCC tissues and cells utilizing TRIzol ® reagent (Invitrogen; Thermo Fisher Scientific, Inc.). .. Complementary DNA (cDNA) synthesis was subsequently performed using GoScript Reverse Transcriptase, adhering to the manufacturer’s protocol (Promega Corporation). .. The qPCR amplification was carried out using GoTaq qPCR Master Mix (Promega Corporation).

Article Title: CMTR1-catalyzed 2'-O-methylation Promotes NMDA Receptor Signaling, Long-term Potentiation and Memory.
Article Snippet: .. The resulting RNA pellet was dissolved in preheated ultrapure water, centrifuged twice at 12,000 xg for 1 min at 4°C to elute the RNA, and quantified with a NanoDrop 1000 spectrophotometer (Thermo Fisher). cDNA synthesis was performed using GoScript reverse transcriptase (Promega) and random primers. qPCR was conducted on a Light Cycler 480 system (Roche) with the Universal Probe Library (UPL) reagent, using Gapdh mRNA as a reference to calculate relative expression levels of target genes via the comparative threshold cycle method. ..

Article Title: Unveiling Crocosphaera Responses to Phosphorus Depletion: Insights From Genome Analysis and Functional Characterization.
Article Snippet: RNAs were quantified spectrophotometrically at 260 nm (NanoDrop 1000; Thermo Fisher Scientific). .. For cDNA synthesis, 200 ng total RNA and 0.5 μg Random Primers (Promega) were used with the GoScript Reverse Transcriptase (Promega) according to the manufacturer's instructions. .. Quantitative real- time PCR (qPCR) analyses were performed on a CFX96 Real- Time System (Bio- Rad).

Article Title: Impact of acute environmental challenges on the expression of peptide hormone-encoding genes in the zebrafish caudal neurosecretory system.
Article Snippet: The integrity of RNA was verified by electrophoresis on 1% agarose gel, and RNA concentration was determined using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific). .. Then, Jo ur na l P re -p ro of Journal Pre-proof 10 cDNAs were obtained using 500 ng of RNA with GoScript reverse transcriptase (Promega – Lyon, France) and random primers (Promega). cDNAs were diluted 10-fold prior to real-time PCR. .. Quantification was carried out by real-time PCR using specific primer pairs and PowerUp SYBR Green (Applied Biosystems, Thermo Fisher Scientific) on a QuantStudioTM 6 Flex RealTime PCR System (QuantStudioTM 6 Flex, Applied Biosystems, Thermo Fisher Scientific).

Polymerase Chain Reaction:

Article Title: Identification of a novel hypovirulence-inducing ourmia-like mycovirus from Fusarium solani causing ginseng ( Panax ginseng ) root rot
Article Snippet: This dsRNA was then treated with enzymes including S1 Nuclease and Recombinant DNase I (TaKaRa, Dalian, China) before electrophoresis on a 1.0% agarose gel. .. For RT-PCR detection, first-strand cDNA was synthesized using GoScript Reverse Transcriptase (Promega, United States), followed by PCR amplification with specific primers (2-4F and 2-4R), as detailed in . ..

Amplification:

Article Title: Identification of a novel hypovirulence-inducing ourmia-like mycovirus from Fusarium solani causing ginseng ( Panax ginseng ) root rot
Article Snippet: This dsRNA was then treated with enzymes including S1 Nuclease and Recombinant DNase I (TaKaRa, Dalian, China) before electrophoresis on a 1.0% agarose gel. .. For RT-PCR detection, first-strand cDNA was synthesized using GoScript Reverse Transcriptase (Promega, United States), followed by PCR amplification with specific primers (2-4F and 2-4R), as detailed in . ..

Expressing:

Article Title: Retinal Angiogenesis in Methamphetamine Self-Administration Rats.
Article Snippet: Relative levels of protein expression normalized with β-actin (Santa Cruz Biotechnology, Dallas, TX, USA) were analyzed by ImageJ, v1.52a (National Institutes of Health, Bethesda, MD, USA). .. To determine mRNA expression levels, total RNA annealed with an oligo dT15 probe was reverse-transcribed to cDNA with GoScript Reverse transcriptase (Promega, Madison, WI, USA). .. The relative mRNA expression levels were estimated using a Bio-Rad CFX Fast Real-Time PCR System and iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: CMTR1-catalyzed 2'-O-methylation Promotes NMDA Receptor Signaling, Long-term Potentiation and Memory.
Article Snippet: .. The resulting RNA pellet was dissolved in preheated ultrapure water, centrifuged twice at 12,000 xg for 1 min at 4°C to elute the RNA, and quantified with a NanoDrop 1000 spectrophotometer (Thermo Fisher). cDNA synthesis was performed using GoScript reverse transcriptase (Promega) and random primers. qPCR was conducted on a Light Cycler 480 system (Roche) with the Universal Probe Library (UPL) reagent, using Gapdh mRNA as a reference to calculate relative expression levels of target genes via the comparative threshold cycle method. ..

cDNA Synthesis:

Article Title: EIF4A3-induced circPRKAR1B promotes esophageal squamous cell carcinoma progression through binding PKM2 to regulate NF-κB induced CCL3 secretion
Article Snippet: Total RNA was extracted from ESCC tissues and cells utilizing TRIzol ® reagent (Invitrogen; Thermo Fisher Scientific, Inc.). .. Complementary DNA (cDNA) synthesis was subsequently performed using GoScript Reverse Transcriptase, adhering to the manufacturer’s protocol (Promega Corporation). .. The qPCR amplification was carried out using GoTaq qPCR Master Mix (Promega Corporation).

Article Title: CMTR1-catalyzed 2'-O-methylation Promotes NMDA Receptor Signaling, Long-term Potentiation and Memory.
Article Snippet: .. The resulting RNA pellet was dissolved in preheated ultrapure water, centrifuged twice at 12,000 xg for 1 min at 4°C to elute the RNA, and quantified with a NanoDrop 1000 spectrophotometer (Thermo Fisher). cDNA synthesis was performed using GoScript reverse transcriptase (Promega) and random primers. qPCR was conducted on a Light Cycler 480 system (Roche) with the Universal Probe Library (UPL) reagent, using Gapdh mRNA as a reference to calculate relative expression levels of target genes via the comparative threshold cycle method. ..

Article Title: Unveiling Crocosphaera Responses to Phosphorus Depletion: Insights From Genome Analysis and Functional Characterization.
Article Snippet: RNAs were quantified spectrophotometrically at 260 nm (NanoDrop 1000; Thermo Fisher Scientific). .. For cDNA synthesis, 200 ng total RNA and 0.5 μg Random Primers (Promega) were used with the GoScript Reverse Transcriptase (Promega) according to the manufacturer's instructions. .. Quantitative real- time PCR (qPCR) analyses were performed on a CFX96 Real- Time System (Bio- Rad).

Spectrophotometry:

Article Title: CMTR1-catalyzed 2'-O-methylation Promotes NMDA Receptor Signaling, Long-term Potentiation and Memory.
Article Snippet: .. The resulting RNA pellet was dissolved in preheated ultrapure water, centrifuged twice at 12,000 xg for 1 min at 4°C to elute the RNA, and quantified with a NanoDrop 1000 spectrophotometer (Thermo Fisher). cDNA synthesis was performed using GoScript reverse transcriptase (Promega) and random primers. qPCR was conducted on a Light Cycler 480 system (Roche) with the Universal Probe Library (UPL) reagent, using Gapdh mRNA as a reference to calculate relative expression levels of target genes via the comparative threshold cycle method. ..

Real-time Polymerase Chain Reaction:

Article Title: CMTR1-catalyzed 2'-O-methylation Promotes NMDA Receptor Signaling, Long-term Potentiation and Memory.
Article Snippet: .. The resulting RNA pellet was dissolved in preheated ultrapure water, centrifuged twice at 12,000 xg for 1 min at 4°C to elute the RNA, and quantified with a NanoDrop 1000 spectrophotometer (Thermo Fisher). cDNA synthesis was performed using GoScript reverse transcriptase (Promega) and random primers. qPCR was conducted on a Light Cycler 480 system (Roche) with the Universal Probe Library (UPL) reagent, using Gapdh mRNA as a reference to calculate relative expression levels of target genes via the comparative threshold cycle method. ..

Article Title: Impact of acute environmental challenges on the expression of peptide hormone-encoding genes in the zebrafish caudal neurosecretory system.
Article Snippet: The integrity of RNA was verified by electrophoresis on 1% agarose gel, and RNA concentration was determined using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific). .. Then, Jo ur na l P re -p ro of Journal Pre-proof 10 cDNAs were obtained using 500 ng of RNA with GoScript reverse transcriptase (Promega – Lyon, France) and random primers (Promega). cDNAs were diluted 10-fold prior to real-time PCR. .. Quantification was carried out by real-time PCR using specific primer pairs and PowerUp SYBR Green (Applied Biosystems, Thermo Fisher Scientific) on a QuantStudioTM 6 Flex RealTime PCR System (QuantStudioTM 6 Flex, Applied Biosystems, Thermo Fisher Scientific).



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